小鼠抗聚组氨酸单克隆抗体-抗体-抗体-生物在线
上海沪震实业有限公司
小鼠抗聚组氨酸单克隆抗体

小鼠抗聚组氨酸单克隆抗体

商家询价

产品名称: 小鼠抗聚组氨酸单克隆抗体

英文名称: His tag(2D5)

产品编号: hzm-0287M

产品价格: null

产品产地: 中国/上海

品牌商标: HZbscience

更新时间: 2023-08-17T10:24:20

使用范围: WB=1:500-5000

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 Mouse Anti-His tag(2D5) antibody

 

产品编号 hzm-0287M
英文名称 His tag(2D5)
中文名称 小鼠抗聚组氨酸单克隆抗体
别    名 HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.  
 
 
 
   
产品类型 标签抗体 
研究领域 细胞生物  免疫学  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 2D5
交叉反应 fusionprotein,HHHHHHtagged
产品应用 WB=1:500-5000 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated HHHHHH peptide:HHHHHH 
亚    型 IgG
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. 

SWISS:
N/A

Gene ID:
N/A

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白

1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。
2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: 细菌,杆状病毒和哺乳动物)
4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌。
5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。
产品图片
Sample: 
His tag protein (Human) at 100 ng
His tag protein (Human) at 100 ng
His tag protein (Human) at 100 ng
Primary: Anti- His tag(2D5) (bsm-0287M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD