研究使用平台：NMT重金属创新科研平台

期刊：Journal of Hazardous Materials

主题：NMT证实豌豆L3变种分生/伸长/成熟区Cd吸收弱于ZM变种

标题：The root iron transporter 1 governs cadmium uptake in Vicia sativa roots

影响因子：7.65

检测指标：Cd²⁺流速

检测样品：豌豆

Cd2+流实验处理方法：

1）10μmolL^-1 Cd预处理10分钟后，检测距离根尖0、0.1、0.2、2、5、10、20mm的根上的点

2）+Cd、+Cd+Fe环境下，检测Cd²⁺流

Cd2+流实验测试液成份：

0.1 mmol L⁻¹ KCl, 0.2 mmol L⁻¹ Na2SO4, and 0.3 mmol L⁻¹ MES, at pH 5.8

作者：南京农业大学沈振国、夏妍、张兴兴

中文摘要（谷歌机翻）

镉是植物的非必需元素，会抑制植物的生长和发育。豌豆的ZM变种比L3变种对Cd毒性更敏感，但其潜在机理尚不完全清楚。

在这里，我们证明根系原生质体中根部Cd含量和Cd荧光强度显示，其Cd积累量高于L3。VsRIT1是ZIP（ZRT / IRT样蛋白）家族的成员，在暴露于Cd的情况下，其ZM根的表达水平比L3根高8倍。VsRIT1表达增加了拟南芥和酵母中Cd的运输和积累。这表明VsRIT1参与了苜蓿根系对镉的吸收。

此外，由于ZM中较高的RIT1表达，当单独暴露于Cd或同时暴露于Cd和铁（Fe）时，ZM根尖具有比L3根更高的瞬时Cd流入能力。我们的发现还表示，镉可能会与铁或锌竞争通过VsRIT1吸收到豌豆或酵母中。

Fig. 6 Fe reduced Cd influx in root tips in ZM and L3. （A)）The transient Cd fluxes of ZM and L3 roots were determined over 10 min after 10 μmol L⁻¹ Cd pre-treatment for 10 min at different distances from the root apex. Negative values indicate Cd influx. (B and C) Fe supply prevents transient Cd influx in both L3 and ZM root tips, columns labelled with distinct lowercase letters indicate statistically significant differences among treatments.

英文摘要

Cadmium is a non-essential element for plants and that inhibits plant growth and development. The ZM variety of Vicia sativa is more sensitive to Cd toxicity than that L3 variety, but the underlying mechanism is not fully understood.

Here, we demonstrated that showed higher Cd accumulation than L3 based on root Cd content and Cd fluorescence intensity in root protoplasts. VsRIT1, a member of ZIP the (ZRT/IRT-like protein) family, showed expression levels in ZM roots 8-fold higher than those in L3 roots under Cd exposure. VsRIT1 expression increased Cd transport and accumulation in Arabidopsis and yeasts. This suggests that VsRIT1 participates in Cd uptake by V. sativa roots.

Furthermore, ZM root tips have a higher capacity for transient Cd influx than L3 roots when exposed to Cd alone or Cd and iron (Fe) together, owing to the higher RIT1 expression in ZM. Our findings also imply that Cd may compete with Fe or/and zinc (Zn) for uptake via VsRIT1 in V. sativa or yeasts.

关键词：非损伤微测技术，Cd²⁺流速，植物重金属，豌豆