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Hepatocyte Growth Factor Activator ( HGFA ) Antibody Datasheet
 
 
 
HGFA / HGF activator抗体产品信息
 
 
 
 
Immunogen: 
 
 
Recombinant human HGFA protein ( Catalog#10329-H08H )
 
 
 
 
 
Clone ID:
 
 
 
10F2C12A5
 
 
 
 
 Ig Type:
 
 
Mouse  IgG1
 
 
 
 
缓冲液:
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
Preparation: 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human HGFA (rhHGFA; Catalog#10329-H08H; NP_001519.1; Met 1- Ser 655). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
HGFA / HGF activator抗体使用指南
 
 
 
 
Specificity: 
 
 
Human  HGFA / HGF activator
 
 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
 
Western blot : 
 
This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect human HGFA in WB. Using a DAB detection system, the detection limit for human HGFA is approximately 8ng/lane under non-reducing conditions and 1ng/lane under reducing conditions.
 
 
 
储存方法:
 
This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 
 
 
 
 HGFA / HGF activator Antibody Related Products & Topics
 
 
相关研究领域:
 
Enzyme>>Protease & Regulator>>Serine Protease & Regulator>>HGFA/HGF Activator
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
HGFA/HGF Activator
 
Human
 
HGFA/HGF Activator Protein, Recombinant  
 
10329-H08H
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Human
 HGFA/HGF Activator
 
ELISA
 
Mouse Monoclonal Antibody
 
10329-MM01
 
 
 
Human
 HGFA/HGF Activator
 
ELISA
 
Mouse Monoclonal Antibody
 
10329-MM02
 
 
 
Human 
 HGFA/HGF Activator
 
ELISA
 
Rabbit Polyclonal Antibody
 
10329-RP02
 
 
 
Human 
 HGFA/HGF Activator
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
10329-RP03
 
 
 
 
 
 HGFA / HGF activator Antibody Background
 
 
HGF activator, also known as HGFA, is a serum-derived serine protease which belongs to the peptidase family S1.HGFA is responsible for the conversion of hepatocyte growth factor (HGF), from the inactive single-chain precursor to the active heterodimeric form, which is a potent mitogen, motogen, and morphogen for liver cells, epithelial cells, and endothelial cells. HGFA is synthesized and secreted by the liver and circulates in the plasma as an inactive single-chain zymogen in normal states. The zymogen is cleaved by thrombin or thermolysin through the endoproteolytic process and forms an active heterodimer linked by a disulfide bond. In turn, the active protease can be inhibited by HGFA inhibitors (HAIs) including HAI-1 and HAI-2. In addition, the HGFA zymogen acquires a strong affinity for heparin upon activation and thus may ensure the local action in tissue regeneration in liver, kidney and skin. It has been reported that activation of HGF is a critical limiting step in the HGF/SF-induced signaling pathway mediated by Met, and accordingly, aberrant expression of HGFA is implicated in tumorigenesis and progression.
 
 
参考文献
 
 
1．Shimomura, T. et al., 1993, J. Biol. Chem. 268: 22927-32.
 
2．Miyazawa, K. et al., 1996, J. Biol. Chem. 271 : 3615-18.
 
3．Shia, S. et al., 2005, J. Mol. Biol. 346: 1335-49.
 
4．Kataoka, H. et al., 2000, J. Biol. Chem. 275: 40453-62.
 
5．Tjin, EP. et al., 2006, Blood. 107: 760-68.
 
6．Kitajima, Y. et al., 2000, Cancer. Res. 60: 6148-59.
 
 
 
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